Recycling/Petri Dishes
From Kimmel Lab Wiki
Contents |
Reusing 100 mm diameter Falcon #3003 polystyrene Petri dishes
Rules for saving used dishes
- The dishes have had only fish, embryo medium and/or fish water in them. No contaminants such as PTU, vital dyes or pharmaceutical agents have been added to the medium.
- There is no writing on the dishes or lids. On the other hand, if you use a Petri dish with a contaminant, please write this on the dish and lid with sharpie.
- There is nothing like dried fish or debris stuck on the dishes.
Bleach bucket solution (0.003% sodium hypochlorite)
For a 10L carboy:
Add 6 ml of 5.25% sodium hypochlorite. Fill to 10 L with tap water. (Run tap until water runs clear of dissolved minerals).
Protocol
- Place the dishes in the dish bleach bucket and the lids in the lid bleach bucket on the Petri dish cart.
- Soak Petri dishes/lids a minimum of 1 hour.
- Putting a lid on the bucket with a sign saying “Add no new dishes” at least an hour before rinsing will assure all have had at least an hour in bleach.
- Rinse individual dishes and lids one by one under running tap water and place in a bucket of clean water. Soak for about one hour in with tap running into bucket; bleach residue is bad for fish.
- Take out the dishes and lids and spread them out on a clean drying rack. When dry, stack in a Petri dish rack labeled “Dishes for Re-Use”.
Sample Sign
For plastic lined container in egg sorting room for researchers to put used dishes:
USED PETRI DISHES, 100 MM DIAMETER (NALGENE #3003) THAT HAVE HAD ONLY FISH, EMBRYO MEDIUM &/OR FISH WATER
NO CONTAMINANTS (EG. PTU, VITAL DYES, DRUGS)
NO WRITING, use removable tape for labeling
If you use anything in addition to the EM, such as PTU, PLEASE WRITE THIS ON THE DISH AND THE LID
