Recipes/Lysis
From Kimmel Lab Wiki
Lysis
| For 50 ml | Ingredient | Location |
|---|---|---|
| 500 µl | 1MTris, pH 8.3 | Area D, Shelf 3 |
| 2500 µl | 1M KCl | Area C, Shelf 4 |
| 75 µl | 1M MgCl2 | Area D, Shelf 2 |
| 750 µl | 20% Tween-20 | Area C, Shelf 3 |
| 1500 µl | 10% NP40 (also known as Igepal) (or Nonidet p40 Roche 11 332 473 001 10 ml bottle; 10% solution) | Area M, Shelf 3 on the left |
Direction
- 50 µl of lysis buffer per well of 96 well plate, add identified fish
- Use long lysis procedure ~3.5 hours
- 5 µl of 10 mg/ml ProK per well.
