Recipes/20XEM

From Kimmel Lab Wiki

20X E.M. Stock

1 L	Ingredients	Locations
17.5 g	NaCl	Area C, Shelf 4
0.75 g	KCl	Area C, Shelf 4
2.9 g	CaCl2 • 2H20	Area C, Shelf 4
0.41 g	KH2PO4	Area C, Shelf 4
0.142 g	NA2HPO4 (Anhy)	Area C, Shelf 4
4.9 g	MgSO4 • 7H20	Area C, Shelf 4
to 1000 ml	Distilled Water	Area H, Shelf 4

Directions

1. Get a 250 ml Graduated Cylinder (Cabinet 5).
2. Measure NaCl, KCl and CaCl₂ • 2H₂0 into the cylinder.
3. Added Water to 250 ml and pour into a fish-safe, 1 L container (Cabinet 5).
4. Pour 250 ml of Water into 1 L container.
5. Measure KH₂PO₄,NA₂HPO₄ and MgSO₄ • 7H₂0 into the cylinder.
6. Repeat Step 3 and 4.
7. Place on a stir plate until fully disolved.
8. Filter using a Nalgene Filter (Cabinet 1).